Lamp is an isothermal nucleic acid amplification technique. Reverse transcription loop mediated isothermal amplification rt lamp combines lamp with a reverse transcription step to allow the detection of rna.
Loop mediated isothermal amplification lamp overview.
Loop mediated isothermal amplification lamp. Using specially designed primers the polymerase produces products with loop structures. Lamp provides high sensitivity to fg or 10 copies of target but with rapid results. Loop mediated isothermal amplification lamp was designed using the bst polymerase which is a polymerase that has high strand displacement activity to amplify target dna or rna once it has been converted to dna by a reverse transcriptase step.
Loop mediated isothermal amplification lamp is a single tube technique for the amplification of dna and a low cost alternative to detect certain diseases. A strand displacing dna polymerase initiates synthesis and 2 specially designed primers form loop structures to facilitate subsequent rounds of amplification through extension on the loops and additional annealing of primers. Loop mediated isothermal amplification lamp is well known for its robust and highly sensitive and specific amplification of target dna which is achieved by utilizing up to six primers.
Lamp is an isothermal amplification method designed to detect a target nucleic acid without requiring sophisticated equipment. Loop mediated isothermal amplification lamp uses 4 6 primers recognizing 6 8 distinct regions of target dna for a highly specific amplification reaction. Reactions can be performed in as little as 5 10 minutes.
Moreover lamp excels through its isothermal and energy efficient amplification requirements rendering it a prime candidate for low cost diagnostics and analysis at the point of need.